Liang Y, et al. (J Biol Chem. 1999) reported that five known rat and human Slit proteins contain 1523-1534 amino acids, and peptide sequences correspond best to those present in human Slit-1 and Slit-2. Binding of these ligands to the glypican-Fc fusion protein requires the presence of the heparan sulfate chains, but the interaction appears to be relatively specific for glypican-1 insofar as no other identified heparin-binding proteins were isolated using our affinity matrix. And they stated that Slit1 and Slit2 are functional ligands of glypican-1 in nervous tissue.
Brose K,et al. (Cell. 1999) characterized three mammalian Slit homologs and showed that the Drosophila Slit protein and at least one of the mammalian Slit proteins, Slit2, are proteolytically processed and show specific, high-affinity binding to Robo proteins (Robo1 and Robo2).Syndecan is a necessary component of Slit/Robo signaling and is required in the Slit target cells (Steigemann et al. 2004).