Liang Y, et al. (J Biol Chem. 1999)
reported that five known rat and human Slit proteins contain 1523-1534 amino acids, and peptide sequences correspond best to those present in human Slit-1 and Slit-2. Binding of these ligands to the glypican-Fc fusion protein requires the presence of the heparan sulfate chains, but the interaction appears to be relatively specific for glypican-1 insofar as no other identified heparin-binding proteins were isolated using our affinity matrix. And they stated that Slit1 and Slit2 are functional ligands of glypican-1 in nervous tissue.